fatty acid oxidation detection reagent Search Results


gene exp ptgs2 mm00478374 m1  (Thermo Fisher)
99
Thermo Fisher gene exp ptgs2 mm00478374 m1
Gene Exp Ptgs2 Mm00478374 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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oadc  (Chem Impex International)
95
Chem Impex International oadc
Oadc, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pediatric mitochondrial trifunctional protein defects screening  (ProSpec)
90
ProSpec pediatric mitochondrial trifunctional protein defects screening
Pediatric Mitochondrial Trifunctional Protein Defects Screening, supplied by ProSpec, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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b-oxidation pathway  (Pfleger GmbH)
90
Pfleger GmbH b-oxidation pathway
B Oxidation Pathway, supplied by Pfleger GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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30 m nitroterephthalic acid modified polyethylene glycol (peg) polymer capillary column 007-ffap  (Quadrex Inc)
90
Quadrex Inc 30 m nitroterephthalic acid modified polyethylene glycol (peg) polymer capillary column 007-ffap
30 M Nitroterephthalic Acid Modified Polyethylene Glycol (Peg) Polymer Capillary Column 007 Ffap, supplied by Quadrex Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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oxylipins and deuterated standards  (Cayman Chemical)
90
Cayman Chemical oxylipins and deuterated standards
Oxylipins And Deuterated Standards, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nicotinamide adenine dinucleotide nad nadh assay kit  (Abcam)
94
Abcam nicotinamide adenine dinucleotide nad nadh assay kit
Melatonin‐induced change in Cal‐27 cell metabolism by upregulation of fatty acid oxidation and TCA metabolites. (A–D) Metabolomic study of intracellular levels of (A) succinic acid, (B) fumaric acid, (C) malic acid, and (D) ketoglutaric acid. (E) <t>NADH</t> and <t>NAD+</t> levels measured using a colorimetric test and expressed as a ratio. (F–I) Western blot analysis of (F) MDAC, (G) EHHADH, and (H) PACC/ACC ratio. (J–M) Evaluation of SC formation by BNGE. Treatment groups included vehicle (control) and melatonin (aMT) at 500 or 1000 µM for 24 h (gray range) or 48 h (blue range) in Cal‐27 cells. Data are presented as mean ± standard error of the mean ( n = 3–7 for each group; one‐tailed unpaired t ‐test; * p < .05; ** p < .01; *** p < .001 vs. control). BNGE, blue native gel electrophoresis; EHHADH, enoyl‐CoA hydratase and 3‐hydroxyacyl CoA dehydrogenase; PACC/ACC, phosphorylated/no‐ phosphorylated acetyl‐CoA carboxylase; SC, supercomplex; TCA, tricarboxylic acid.
Nicotinamide Adenine Dinucleotide Nad Nadh Assay Kit, supplied by Abcam, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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colorimetric assay kit  (Elabscience Biotechnology)
94
Elabscience Biotechnology colorimetric assay kit
Melatonin‐induced change in Cal‐27 cell metabolism by upregulation of fatty acid oxidation and TCA metabolites. (A–D) Metabolomic study of intracellular levels of (A) succinic acid, (B) fumaric acid, (C) malic acid, and (D) ketoglutaric acid. (E) <t>NADH</t> and <t>NAD+</t> levels measured using a colorimetric test and expressed as a ratio. (F–I) Western blot analysis of (F) MDAC, (G) EHHADH, and (H) PACC/ACC ratio. (J–M) Evaluation of SC formation by BNGE. Treatment groups included vehicle (control) and melatonin (aMT) at 500 or 1000 µM for 24 h (gray range) or 48 h (blue range) in Cal‐27 cells. Data are presented as mean ± standard error of the mean ( n = 3–7 for each group; one‐tailed unpaired t ‐test; * p < .05; ** p < .01; *** p < .001 vs. control). BNGE, blue native gel electrophoresis; EHHADH, enoyl‐CoA hydratase and 3‐hydroxyacyl CoA dehydrogenase; PACC/ACC, phosphorylated/no‐ phosphorylated acetyl‐CoA carboxylase; SC, supercomplex; TCA, tricarboxylic acid.
Colorimetric Assay Kit, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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polyoxyethylene sorbitan fatty acid esters (tween®  (ICI Americas Inc)
90
ICI Americas Inc polyoxyethylene sorbitan fatty acid esters (tween®
Melatonin‐induced change in Cal‐27 cell metabolism by upregulation of fatty acid oxidation and TCA metabolites. (A–D) Metabolomic study of intracellular levels of (A) succinic acid, (B) fumaric acid, (C) malic acid, and (D) ketoglutaric acid. (E) <t>NADH</t> and <t>NAD+</t> levels measured using a colorimetric test and expressed as a ratio. (F–I) Western blot analysis of (F) MDAC, (G) EHHADH, and (H) PACC/ACC ratio. (J–M) Evaluation of SC formation by BNGE. Treatment groups included vehicle (control) and melatonin (aMT) at 500 or 1000 µM for 24 h (gray range) or 48 h (blue range) in Cal‐27 cells. Data are presented as mean ± standard error of the mean ( n = 3–7 for each group; one‐tailed unpaired t ‐test; * p < .05; ** p < .01; *** p < .001 vs. control). BNGE, blue native gel electrophoresis; EHHADH, enoyl‐CoA hydratase and 3‐hydroxyacyl CoA dehydrogenase; PACC/ACC, phosphorylated/no‐ phosphorylated acetyl‐CoA carboxylase; SC, supercomplex; TCA, tricarboxylic acid.
Polyoxyethylene Sorbitan Fatty Acid Esters (Tween®, supplied by ICI Americas Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tween series of surfactants  (ICI Americas Inc)
90
ICI Americas Inc tween series of surfactants
Melatonin‐induced change in Cal‐27 cell metabolism by upregulation of fatty acid oxidation and TCA metabolites. (A–D) Metabolomic study of intracellular levels of (A) succinic acid, (B) fumaric acid, (C) malic acid, and (D) ketoglutaric acid. (E) <t>NADH</t> and <t>NAD+</t> levels measured using a colorimetric test and expressed as a ratio. (F–I) Western blot analysis of (F) MDAC, (G) EHHADH, and (H) PACC/ACC ratio. (J–M) Evaluation of SC formation by BNGE. Treatment groups included vehicle (control) and melatonin (aMT) at 500 or 1000 µM for 24 h (gray range) or 48 h (blue range) in Cal‐27 cells. Data are presented as mean ± standard error of the mean ( n = 3–7 for each group; one‐tailed unpaired t ‐test; * p < .05; ** p < .01; *** p < .001 vs. control). BNGE, blue native gel electrophoresis; EHHADH, enoyl‐CoA hydratase and 3‐hydroxyacyl CoA dehydrogenase; PACC/ACC, phosphorylated/no‐ phosphorylated acetyl‐CoA carboxylase; SC, supercomplex; TCA, tricarboxylic acid.
Tween Series Of Surfactants, supplied by ICI Americas Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mapeg® polyethylene glycol esters  (BASF)
90
BASF mapeg® polyethylene glycol esters
Melatonin‐induced change in Cal‐27 cell metabolism by upregulation of fatty acid oxidation and TCA metabolites. (A–D) Metabolomic study of intracellular levels of (A) succinic acid, (B) fumaric acid, (C) malic acid, and (D) ketoglutaric acid. (E) <t>NADH</t> and <t>NAD+</t> levels measured using a colorimetric test and expressed as a ratio. (F–I) Western blot analysis of (F) MDAC, (G) EHHADH, and (H) PACC/ACC ratio. (J–M) Evaluation of SC formation by BNGE. Treatment groups included vehicle (control) and melatonin (aMT) at 500 or 1000 µM for 24 h (gray range) or 48 h (blue range) in Cal‐27 cells. Data are presented as mean ± standard error of the mean ( n = 3–7 for each group; one‐tailed unpaired t ‐test; * p < .05; ** p < .01; *** p < .001 vs. control). BNGE, blue native gel electrophoresis; EHHADH, enoyl‐CoA hydratase and 3‐hydroxyacyl CoA dehydrogenase; PACC/ACC, phosphorylated/no‐ phosphorylated acetyl‐CoA carboxylase; SC, supercomplex; TCA, tricarboxylic acid.
Mapeg® Polyethylene Glycol Esters, supplied by BASF, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tween 80 polyethylene glycol sorbate monooleate  (Merck KGaA)
90
Merck KGaA tween 80 polyethylene glycol sorbate monooleate
Melatonin‐induced change in Cal‐27 cell metabolism by upregulation of fatty acid oxidation and TCA metabolites. (A–D) Metabolomic study of intracellular levels of (A) succinic acid, (B) fumaric acid, (C) malic acid, and (D) ketoglutaric acid. (E) <t>NADH</t> and <t>NAD+</t> levels measured using a colorimetric test and expressed as a ratio. (F–I) Western blot analysis of (F) MDAC, (G) EHHADH, and (H) PACC/ACC ratio. (J–M) Evaluation of SC formation by BNGE. Treatment groups included vehicle (control) and melatonin (aMT) at 500 or 1000 µM for 24 h (gray range) or 48 h (blue range) in Cal‐27 cells. Data are presented as mean ± standard error of the mean ( n = 3–7 for each group; one‐tailed unpaired t ‐test; * p < .05; ** p < .01; *** p < .001 vs. control). BNGE, blue native gel electrophoresis; EHHADH, enoyl‐CoA hydratase and 3‐hydroxyacyl CoA dehydrogenase; PACC/ACC, phosphorylated/no‐ phosphorylated acetyl‐CoA carboxylase; SC, supercomplex; TCA, tricarboxylic acid.
Tween 80 Polyethylene Glycol Sorbate Monooleate, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Melatonin‐induced change in Cal‐27 cell metabolism by upregulation of fatty acid oxidation and TCA metabolites. (A–D) Metabolomic study of intracellular levels of (A) succinic acid, (B) fumaric acid, (C) malic acid, and (D) ketoglutaric acid. (E) NADH and NAD+ levels measured using a colorimetric test and expressed as a ratio. (F–I) Western blot analysis of (F) MDAC, (G) EHHADH, and (H) PACC/ACC ratio. (J–M) Evaluation of SC formation by BNGE. Treatment groups included vehicle (control) and melatonin (aMT) at 500 or 1000 µM for 24 h (gray range) or 48 h (blue range) in Cal‐27 cells. Data are presented as mean ± standard error of the mean ( n = 3–7 for each group; one‐tailed unpaired t ‐test; * p < .05; ** p < .01; *** p < .001 vs. control). BNGE, blue native gel electrophoresis; EHHADH, enoyl‐CoA hydratase and 3‐hydroxyacyl CoA dehydrogenase; PACC/ACC, phosphorylated/no‐ phosphorylated acetyl‐CoA carboxylase; SC, supercomplex; TCA, tricarboxylic acid.

Journal: Journal of Pineal Research

Article Title: Melatonin drives apoptosis in head and neck cancer by increasing mitochondrial ROS generated via reverse electron transport

doi: 10.1111/jpi.12824

Figure Lengend Snippet: Melatonin‐induced change in Cal‐27 cell metabolism by upregulation of fatty acid oxidation and TCA metabolites. (A–D) Metabolomic study of intracellular levels of (A) succinic acid, (B) fumaric acid, (C) malic acid, and (D) ketoglutaric acid. (E) NADH and NAD+ levels measured using a colorimetric test and expressed as a ratio. (F–I) Western blot analysis of (F) MDAC, (G) EHHADH, and (H) PACC/ACC ratio. (J–M) Evaluation of SC formation by BNGE. Treatment groups included vehicle (control) and melatonin (aMT) at 500 or 1000 µM for 24 h (gray range) or 48 h (blue range) in Cal‐27 cells. Data are presented as mean ± standard error of the mean ( n = 3–7 for each group; one‐tailed unpaired t ‐test; * p < .05; ** p < .01; *** p < .001 vs. control). BNGE, blue native gel electrophoresis; EHHADH, enoyl‐CoA hydratase and 3‐hydroxyacyl CoA dehydrogenase; PACC/ACC, phosphorylated/no‐ phosphorylated acetyl‐CoA carboxylase; SC, supercomplex; TCA, tricarboxylic acid.

Article Snippet: NAD+ levels were measured using a oxidized and reduced nicotinamide adenine dinucleotide (NAD/NADH) assay kit (ab65348; Abcam) according to the manufacturer's instructions.

Techniques: Western Blot, One-tailed Test, Nucleic Acid Electrophoresis